Product Introduction:
Protein A/G magnetic beads contain a recombinant Protein A/G that combines the IgG binding domains of both Protein A and Protein G. Protein A/G allows for the capture of immunoglobulins from a broader range of species and antibody isotypes than either Protein A or Protein G alone.
The protein A/G magnetic beads provided by Alfa Chemistry have a large specific surface area, more binding sites, higher antibody binding capacity, and extremely low non-specific binding rates. They are typically used for isolating antibodies from serum, cell culture supernatants, or ascites, as well as for immunoprecipitation and co-immunoprecipitation of antigens from cell or tissue extracts.
| Product Name | Protein A/G Magnetic Beads |
| Catalog Number | NM-MBs052 |
| Particle Size | 2 μm |
| Concentration | 10 mg/mL |
| Preservation Solution | 1×PBS, 0.1% (w/v) BSA, 0.1% (v/v) proclin-300 |
| Binding Capacity | ≥0.4 mg Human IgG/1 mL magnetic beads |
| Storage Condition | Seal and store at 4 °C for 24 months. Do not freeze. Mix well before use. |
*If you have a need for other specifications, we can also provide you with product customization.
The following are only recommended buffer components. You can adjust the buffer formulation as needed. Please note that all reagents need to be prepared using DEPC-treated purified water.
| Problem | Possible Cause | Solution |
| The binding efficiency of the antibody to the magnetic beads is low. | The binding efficiency of the magnetic beads with antibody is related to the species origin and subtype of the antibody. | Check the affinity of antibody and the Protein A/G matrix (see appendix). |
| The antibody subtype has a lower affinity for Protein A/G matrix. | Increase the incubation time of the antibody with the magnetic beads (30-120 min), increase the pH of the binding buffer (8-9) and decrease the ionic strength (25-100 mM NaCl). | |
| Aggregation of magnetic beads occurs during storage or use. | Improper storage of magnetic beads. | Handle the magnetic beads according to the instructions in the manual. |
| Freeze or centrifuge magnetic beads. | ||
| Clumping of magnetic beads occurs during use. | Prolonged placement of the magnetic beads in the magnetic field causes them to firmly bind together. | Ultrasonic water bath treatment for 2 minutes can redisperse the magnetic beads. However, note that ultrasonic treatment may also cause the antibodies captured by the magnetic beads in the sample solution to detach, so this method should not be used after adding the sample and before elution. |
Appendix: Binding Affinity of Protein A/G to Different Species and Subtypes of Antibodies
| Species | Antibody Subtype | Protein A/G |
| Human | Total IgG | +++++ |
| IgG1, IgG2 | +++++ | |
| IgG3 | +++++ | |
| IgG4 | +++++ | |
| IgM | - | |
| IgD | - | |
| IgA | + | |
| IgA1, IgA2 | + | |
| IgE | +++ | |
| Fab | - | |
| ScFv | - | |
| Mouse | Total IgG | +++++ |
| IgM | - | |
| IgG1 | +++ | |
| IgG2a | +++ | |
| IgG2b | +++ | |
| IgG3 | +++ | |
| Rat | Total IgG | +++ |
| IgG1 | +++ | |
| IgG2a | +++++ | |
| IgG2b | + | |
| IgG2c | +++++ | |
| Cow | Total IgG | +++++ |
| IgG1 | +++++ | |
| IgG2 | +++++ | |
| Goat | Total IgG | +++++ |
| IgG1 | +++++ | |
| IgG2 | +++++ | |
| Sheep | Total IgG | +++++ |
| IgG1 | +++++ | |
| IgG2 | +++++ | |
| Horse | Total IgG | +++++ |
| IgG(ab), IgG(c) | + | |
| IgG(T) | +++++ | |
| Rabbit | Total IgG | +++++ |
| Guinea Pig | Total IgG | +++++ |
| Hamster | Total IgG | +++ |
| Pig | Total IgG | +++++ |
| Donkey | Total IgG | +++++ |
Note: "+"=weak binding, "+++"=medium binding, "+++++"=strong binding, "-"=no binding
As a specialist in nanomaterials, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.
101-5 Colin Dr, Holbrook, NY 11741
Tel: 1-516-662-5404
Fax: 1-516-927-0118
Email: info@alfa-chemistry.com
Privacy Policy | Cookie Policy | Copyright © 2025 Alfa Chemistry. All rights reserved.