Flow Cytometry Absolute Counting Microspheres

Product Introduction:
Flow cytometry absolute counting microspheres are a calibrated suspension of microspheres used to accurately determine the absolute count of cells in a sample. These microspheres are usually composed of polystyrene and are uniformly spherical and precisely sized. The application of these microspheres enhances the accuracy and reproducibility of flow cytometry analysis, providing valuable insights into the composition and dynamics of cell populations in biological samples.

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Our Products

Alfa Chemistry offers a range of flow cytometry absolute counting microspheres. They feature a highly uniform particle size distribution and fluorescence intensity distribution (CV< 5%). Unstained absolute counting microspheres can determine the absolute number of cells in a sample via the signal of light scattered by a flow cytometer. Internally stained fluorescent microspheres can generate signals in commonly used fluorescence channels such as PE and can be distinguished from the detected cell population. This allows for the calculation of cell counts by assessing the ratio of the microspheres to the cells. Rainbow flow cytometry absolute counting microspheres generate signals in all commonly used fluorescence channels, making them suitable for cell counting in mmulticolor flow assays. If you are interested in our products and want to know more product information, please feel free to contact us.

Product Name	Stained Flow Cytometry Absolute Counting Microspheres	Rainbow Flow Cytometry Absolute Counting Microspheres	Unstained Flow Cytometry Absolute Counting Microspheres
Catalog Number	NM-MSPs033	NM-MSPs034	NM-MSPs035
Mean Particle Size	5 μm, 7 μm, 9 μm	5 μm, 7 μm, 9 μm	5 μm, 7 μm, 9 μm
Matrix	Polystyrene	Polystyrene	Polystyrene
Pore Structure	Non-porous solid	Non-porous solid	Non-porous solid
Excitation Wavelength	550 nm (maximum excitation wavelength)	488 nm-640 nm (excitation wavelength range)	NA
Emission Wavelength	570 nm (maximum emission wavelength)	520 nm-780 nm (Emission wavelength range)	NA
Available Detection Channels	PE	FSC, SSC, FITC, PE, APC, Cy7	FSC, SSC
Particle Concentration	1×10⁶ beads/mL	1×10⁶ beads/mL	1×10⁶ beads/mL
Density	1.05 g/cm³	1.05 g/cm³	1.05 g/cm³
Refractive Index	1.59@589 nm	1.59@589 nm	1.59@589 nm
Preservation Solution	Pure water (containing 0.05% w/v Proclin 300)	Pure water (containing 0.05% w/v Proclin 300)	Pure water (containing 0.05% w/v Proclin 300)

Product Advantages

Our products provide accurate and reproducible quantification of cells.
Our products simplify the process of obtaining absolute counts compared to traditional manual counting methods.
Our products are available for a wide range of cell types and sample types.

Product Applications

Our products play a crucial role in flow cytometry analysis by providing a reliable method for quantifying cells and enabling researchers to make accurate and informed conclusions based on their experimental data. Specifically, they are particularly useful in monitoring changes in cell populations over time, detecting rare cell populations, tracking disease progression, and evaluating the effectiveness of treatments. They are also used in quality control and standardization of flow cytometry assays to ensure consistent and reliable results.

You Need to Know

Our products need to be sealed and stored at 2-8 °C, do not freeze.
Our products are for scientific research use only and should not be used for other purposes.