Oligo (dT) Magnetic Beads

Product Introduction:
Oligo (dT) magnetic beads are specialized particles designed for the isolation of mRNA from a variety of biological samples. They are coated with oligo (dT) sequences, which are short stretches of deoxythymidine nucleotides. These oligo (dT) sequences can specifically bind to the polyadenylate [poly (A)] tails found at the 3' end of eukaryotic mRNA molecules. Based on these characteristics, Oligo (dT) magnetic beads have become powerful tools in molecular biology for studying gene expression and performing other analyses that require high-quality mRNA.

Specification

Price$590

Quantity

- +

Online Order Contact Us

Our Products

The oligo (dT) magnetic beads provided by Alfa Chemistry are characterized by good dispersibility, rapid magnetic response, and excellent hydrophilicity. Through the complementary pairing between Oligo dT on the surface of magnetic beads and mRNA tail Ploy A, complete and high-purity mRNA can be efficiently and rapidly isolated from eukaryotic total RNA or directly from animal and plant tissues and cells. The isolated mRNA can be used in various downstream molecular biology experiments, such as RT-PCR, solid-phase cDNA library construction, Northern blot analysis, primer extension, and gene expression analysis.

Product Name	1 μm Oligo (dT) Magnetic Beads	2.8 μm Oligo (dT) Magnetic Beads
Catalog Number	NM-MBs050	NM-MBs051
Particle Size	1 μm	2.8 μm
Oligo (dT) Coupling Capacity	~500 pmol/1 mg magnetic beads	~400 pmol/1 mg magnetic beads
mRNA Binding Capacity	1-2 μg/1 mg magnetic beads
Concentration	5 mg/mL
Preservation Solution	1×PBS, 0.01% Tween-20, 0.1% proclin-300
Storage Condition	Seal and store at 4 °C for 24 months. Do not freeze. Mix well before use.

*If you have a need for other specifications, we can also provide you with product customization.

Recommended Buffers

The following are only recommended buffer components. You can adjust the buffer formulation as needed. Please note that all reagents need to be prepared using DEPC-treated purified water.

Binding buffer: 10 mM Tris-HCl (pH 7.5), 1.0 M NaCl, 1 mM EDTA
Lysis/binding buffer: 100 mM Tris-HCl (pH 7.5), 500 mM NaCl, 10 mM EDTA, 1% SDS, 5 mM DTT
Wash buffer I: 10 mM Tris-HCl (pH 7.5), 0.15 M NaCl, 1 mM EDTA, 0.1% SDS
Wash buffer II: 10 mM Tris-HCl (pH 7.5), 0.15 M NaCl, 1 mM EDTA
Elution buffer: Nuclease-free water

You Need to Know

Freezing of magnetic beads or any other inappropriate operation is prohibited.
Before using the magnetic beads, shake them thoroughly to ensure even suspension. Bubbles should be avoided during operation.
In order to reduce the loss of magnetic beads, each magnetic separation time should be no less than 1 min.
If the purified rRNA residue is too much to proceed with downstream applications, it is recommended to perform a second round of mRNA purification using new magnetic beads.
It is advised to use the extracted mRNA for RT-PCR right away. If storage is required, it is recommended to add RNase inhibitor to the eluent.
All buffers and consumables used for mRNA extraction should be RNase-free.
It is recommended to use high-quality pipette tips and reaction tubes to avoid losses due to adhesion of magnetic beads and solution.
Our products are for research use only.