Heparin-Conjugated Agarose Magnetic Beads

Product Introduction:
Heparin-conjugated agarose magnetic beads are specialized bio-functional materials designed for various biomedical and biochemical applications. Heparin is a naturally occurring, highly sulfated glycosaminoglycan. It has a strong affinity for binding various proteins, particularly those involved in blood coagulation and cell signaling. The incorporation of heparin onto the surface of agarose magnetic beads allows for specific binding and purification of heparin-binding proteins or molecules.

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Our Products

The heparin-conjugated agarose magnetic beads provided by Alfa Chemistry have the characteristics of fast magnetic response, abundant heparin density, and extremely high physical and chemical stability. They can be used as ligands for affinity chromatography and are capable of specific binding to biomolecules such as growth factors and antithrombin. Additionally, due to the presence of a large number of negatively charged sulfate ion groups on their surface, our products can also function as a cation exchange medium, binding to positively charged proteins under certain pH conditions.

Compared to traditional column chromatography purification methods, our heparin-conjugated agarose magnetic beads do not require pretreatment of crude protein samples, nor do they require control of flow rate and column pressure. For experienced operators, high-purity target proteins can be extracted in a very short time, and the parallel processing of multiple samples can be easily achieved, enabling high-throughput protein purification.

Product Name	Heparin-Conjugated Agarose Magnetic Beads
Catalog Number	NM-MBs065
Bead Size	30-150 μm
Ligand Density	~3 mg Heparin/1 mL gel
Protein Binding Capacity	2-3 mg Antithrombin III/1 mL gel
Preservation Solution	20% (V/V) Ethanol solution
Suspension Concentration	10% (V/V) Magnetic beads suspension
Binding Buffer	50 mM Tris-HCl, pH 8.0
Elution Buffer	50 mM Tris-HCl, 1-2 M NaCl, pH 8.0
Storage Condition	Seal and store at 4 °C for 24 months. Do not freeze. Mix well before use.

*The amount of protein binding is related to the characteristics of the target protein, and only the reference value is given here.
**If you have a need for other specifications, we can also provide you with product customization.

Advantages

Our products provide sufficient binding sites for biomolecule attachment.
Our products enable fast and efficient separation of target molecules from mixtures using a magnetic field.
Our products have good dispersion and resuspension, and improve the convenience of operation.
The heparin ligand has good physical and chemical stability, which improves the reliability and repeatability of the experimental results.

You Need to Know

Do not freeze, dry, or centrifuge our products. These operations will cause the beads to agglomerate, making them difficult to resuspend and disperse, and will affect the chemical activity of the surface functional groups on the beads.
Before using our products, make sure to thoroughly oscillate or ultrasonic to maintain a uniform suspension of the beads
The magnetic beads can be washed 2 to 3 times with purified water or buffer to remove ethanol from the preservation solution as needed during use.
Salt concentration and pH levels influence the binding and elution of specific proteins. Users must determine the optimal binding and elution conditions for various proteins themselves to guarantee the yield and purity of the protein purification process.
Our products need to be used with magnetic separation equipment.
Our products are for research use only.