His-Tag Protein Purification Magnetic Beads

Product Introduction:
His-tag protein purification magnetic beads are specialized tools used in biochemical and molecular biology labs for the purification of proteins tagged with a series of histidine residues (His-tag). These magnetic beads offer a highly efficient and straightforward method for isolating His-tagged proteins from complex mixtures, such as cell lysates or culture media.

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Our Products

His-tag protein purification magnetic beads provided by Alfa Chemistry are new functional materials designed for efficient and rapid purification of His-tag protein. They can directly purify the target protein from biological samples by magnetic separation, which greatly simplifies the purification process and improves the purification efficiency.

Our products include nickel and cobalt ion-chelated magnetic beads, which differ in their target protein binding capacity and non-specific adsorption properties. Customers can choose different types of metal ion-chelated magnetic beads based on the binding performance of the target protein with different metals, as well as their requirements for yield and purity of the target protein.

Product Name	IDA-Ni Magnetic Beads for His-Tag Protein Purification	IDA-Co Magnetic Beads for His-Tag Protein Purification
Catalog Number	NM-MBs063	NM-MBs064
Bead Size	30-150 µm
Chelating Metal Ion	Ni²⁺	Co²⁺
Metal Ion Density	30-50 µmol/1 mL magnetic beads
Protein Binding Capacity	30-40 mg/mL (100% magnetic beads)	20-30 mg/mL (100% magnetic beads)
Operating Temperature	2-30 °C
Suspension Concentration	10% (V/V) magnetic beads suspension
Preservation Solution	20% (V/V) ethanol solution
Storage Condition	Seal and store at 4 °C for 24 months. Do not freeze. Mix well before use.

*Iminodiacetic acid (IDA) is widely used to fix metal ions to a matrix.
**The amount of protein binding is related to the characteristics of the target protein, and only the reference value is given here.
***If you have a need for other specifications, we can also provide you with product customization.

You Need to Know

Do not freeze, dry, or d high-speed centrifuge our products.
Before using our products, make sure to thoroughly oscillate or ultrasonic to maintain a uniform suspension of the beads.
It is recommended to use high-quality pipette tips and reaction tubes to prevent the magnetic beads from sticking to the walls or leakage during the mixing process, which can cause loss of magnetic beads.
Our products are reusable. When the purification performance decreases, regeneration is recommended.
When the used magnetic beads are reused, it is recommended to purify the same kind of proteins. When purifying different proteins, it is recommended to use new magnetic beads to avoid cross-contamination.
Our products need to be used with magnetic separation equipment.
Our products are for research use only.

Appendix

Appendix: Solvent Tolerance of Magnetic Beads

Solvent Type	Solvent	Tolerable Concentration	Note
Reducing Agent	DTE	5 mM	Before using reductant, please wash the magnetic beads with no reductant solution first. Avoid using reductant solution to treat magnetic beads for a long time.
	DTT	5 mM
	β-Mercaptoethanol	20 mM
	TCEP	5 mM
	Reduced Glutathione	10 mM
Denaturant	Urea	8 M	-
Denaturant	Guanidine Hydrochlride	6 M	-
Surface Active Agent	Triton X-100	2%	-
	Tween 20	2%	-
	NP-40	2%	-
	Cholate	2%	-
	CHAPS	1%	-
Buffer Solution	Sodium Phosphate, pH 7.4	50 mM	-
	HEPES	100 mM	-
	Tris-HCl, pH 7.4	100 mM	-
	Tris-Acetate, pH 7.4	100 mM	-
	MOPS, pH 7.4	100 mM	-
	Sodium Acetate, pH 4.0	100 mM	-
Other Solution	Imidazole	1 M	-
	Ethanol	20%	-
	NaCl	1.5 M	-
	Na₂SO₄	100 mM	-
	Glycerin	50%	-
	EDTA	1 mM	Limited to add to protein samples, not for buffers.
	Citrate	60 mM	Limited to add to protein samples, not for buffers.